Karakterisering Av Läkemedelsresistenta Varianter Av HIV-1 I

Medicinska nyheter från The Pediatric Infectious Disease Journal

Co-receptor tropism testing is also recommended for patients who exhibit virologic failure on a CCR5 inhibitor (BIII). A phenotypic tropism assay (e.g., Trofile) is preferred to determine HIV-1 co-receptor usage (AI). A genotypic tropism assay should be considered as an alternative test to predict HIV-1 co-receptor usage (BII). Test ID BLOD1301 Trofile Co-Receptor Tropism Assay Useful For To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is ≥ 1,000 copies/mL. Monogram Trofile has become, largely by default, the de facto standard for HIV tropism assay. However, there is significant room for improvement in the speed, cost and availability of the test.

Coreceptor tropism assay

The viruses in most (>80%) treatment-naïve patients use the CCR5 (R5) coreceptor.1 Conversely, the viruses in up to 50% of treatment-experienced patients use either the CXCR4 (X4) coreceptor or both coreceptors (ie, R5 and X4).2 Viruses that use both coreceptors are Tropism determination: Coreceptor usage was determined using the Geno2pheno [coreceptor] bioinformatics tool (G2P) 10. False positive rates (FPR) were 10% for the proviral DNA sequences obtained in this study or 20% for clonal and clinical data. The Trofile Coreceptor Tropism Assay (Monogram Biosciences, Data on coreceptor tropism in non‐B HIV‐1 subtypes are limited. We studied coreceptor tropism in HIV‐1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay. Methods. We compiled V3 sequences of HIV‐1 strains circulating in Thailand during 2010–2012.

this project was to study neutralization and coreceptor use in HIV-1 and HIV-2.

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Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.

Information. från Läkemedelsverket - PDF Gratis nedladdning

Tropism refers to the type of cytokine coreceptor used by HIV-1 when infecting the host cell. The viruses in most (>80%) treatment-naïve patients use the CCR5 (R5) coreceptor.1 Conversely, the viruses in up to 50% of treatment-experienced patients use either the CXCR4 (X4) coreceptor or both coreceptors (ie, R5 and X4).2 Viruses that use both coreceptors are CD4 cell culture assay for phenotypic recombinant-virus co-receptor tropism.

Two DNA-based tropism assays are also available. The HIV-1 Coreceptor Tropism, Proviral DNA (Quest Diagnostics) uses population sequencing of the HIV envelope V3 loop to detect the presence of CXCR4-tropic HIV-1 [Baumann et al.
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Strategies to assess viral tropism may be divided into phenotypic and genotypic assays; registrative trials of CCR5 inhibitors used phenotypic assay, but recently genotypic ones 2013-05-08 CORECEPTOR TROPISM ASSAYS HIV-1 can attach to human cells either by using the CCR5 coreceptor, the CXCR4 coreceptor, or both (dual/mixed). Tropism testing determines how the virus can attach to the cells in a given patient. Possible tropism results are R5, DM, X4, and X4 near the limit of detection (NLOD).

To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.
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Information. från Läkemedelsverket - PDF Gratis nedladdning

Test ID BLOD1301 Trofile Co-Receptor Tropism Assay Useful For To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is ≥ 1,000 copies/mL. Monogram Trofile has become, largely by default, the de facto standard for HIV tropism assay. However, there is significant room for improvement in the speed, cost and availability of the test.

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Human Retroviruses – Elisa Vicenzi • Guido Poli – Bok

Methods. We compiled V3 sequences of HIV‐1 strains circulating in Thailand during 2010–2012. Human immunodeficiency virus (HIV) enters and infects target cells by a process involving interactions between the envelope glycoprotein gp120 and the host cell CD4 receptor, then one or both of two chemokine co-receptors CCR5 and CXCR4. HIV strains that use the CCR5 receptor to gain entry are called R5-tropic viruses.